NGS Sample Preparation Market Size, Share, By Offering (Instruments, Consumables, and Services), Workflow (Nucleic Acid Extraction, Library Preparation and Amplification, Target Enrichment, and Library Quantification/QC), Method (Microfluidic, and Automated), Application (Oncology, Clinical Investigation, Reproductive Health, HLA Typing/Immune System Monitoring, Metagenomics, Epidemiology and Drug Development, Agrigenomics and Forensics, and Consumer Genomics), End-User(Academic Research, Clinical Research, Hospitals and Clinics, Pharmaceutical and Biotechnology Entities, and Others), and Region - Trends, Analysis, and Forecast till 2035

• By 2035, the market is expected to reach a valuation of USD 19.5 Billion.
• NGS sample preparation market size was valued at USD 5.0 Billion in 2024.
• Target market is expected to grow at a CAGR of 15.6%.

Biological samples (such as blood or tissue) are prepared for sequencing using Next Generation Sequencing (NGS) technology by extracting DNA or RNA from the sample, fragmenting it, adding the required adapters, and creating a library that can be loaded onto the sequencing platform. This process essentially gets the genetic material ready for high-throughput analysis on an NGS machine. The capability NGS sample preparation to sequence large amounts of genetic material quickly and cost-effectively, which results in high throughput sequencing, detection of rare variants, and the flexibility to target specific regions of interest through targeted sequencing, contributes in propelling the NGS sample preparation market growth.

Moreover, technological advancements in NGS platforms, growing demand of NGS in healthcare, use of NGS in genomic testing, rising prevalence cancer and genetic disorders, and its use in personalized medicine, further leads to market expansion.

Impact of AI on NGS Sample Preparation Market:

AI is essential to the preparation of NGS samples as it automates and optimizes a number of processes, greatly increasing the speed, accuracy, and efficiency of analyzing large genomic datasets. This is especially true when it comes to recognizing patterns and deciphering complex genetic variations, which allows for quicker and more accurate clinical diagnoses and research findings.

Key Drivers of Target Market:

Growing Demand of NGS Sample Preparation in Healthcare is Expected to Skyrocket the Market Growth

NGS sample preparation is an essential step in Next-Generation Sequencing technology, which involves processing biological samples so it is ready for sequencing. As a result of the growing use of NGS in healthcare for infectious disease detection, cancer profiling, genetic disease diagnosis, and personalized medicine, the NGS sample preparation market is expanding significantly.

• For instance, March 2024, the World Health Organization (WHO) had published recommendations on the use of a new class of diagnostic technologies, i.e., targeted NGS tests for the diagnosis of drug-resistant tuberculosis (TB). The recommendations provided a novel approach for the rapid detection of drug resistance to new anti-TB drugs with the use of latest technologies.

Restraints:

Possible Risks of Contamination of the NGS Sample is Obstructing the Market Growth

NGS sample preparation is an essential step that involves extracting, fragmenting, and preparing DNA or RNA for sequencing. However, as it involves multiple handling steps, there is a high risk of contamination, which can result in inaccurate results if not properly managed with strict protocols and proper laboratory practices. Possible sources of contamination include reagents, environmental factors, and cross-contamination between samples.

• Counterbalance Statements: While handling the sample for extraction, fragmentation and preparation of the DNA or RNA sequencing, following certain protocols, such as wearing gloves, laboratory coats, and performing the experiments in a well sanitized laminar air flow can prevent the NGS sample from getting contaminated.

Opportunities:

Increased Automation in Preparation of NGS Sample will Create Significant Opportunities

Robotics systems and automated workflows will be increasingly adopted to minimize the manual errors, improving the efficiency, accuracy, and throughput by minimizing human error and allowing for simultaneous processing of multiple samples. Additionally, increasing the automation in NGS sample preparation will reduce the contamination, and also improve the reproducibility, thereby, leading to further market diversification.

• For instance, in December 2024, QIAGEN has achieved a milestone with over 1,000 placements of its EZ2 Connect automated sample preparation instrument. This advancement automates the entire process of nucleic acid purification, reducing hands-on time and increasing throughput while maintaining high precision and reproducibility. It allows researchers to focus on data analysis and interpretation, particularly for laboratories handling medium volumes of samples for molecular biology research applications like digital PCR and next-generation sequencing.

The market is segmented into offering, workflow, method, application, end-user, and region.

Product

Based on offering, the market is categorized into instruments, consumables, and services. The consumables segment is dominating the NGS sample preparation market share. This is due to the growing demand for consumables in research, diagnostic, and commercial applications. In addition to this, the demand for consumables are increasing with the rising demand for testing and related components and equipment.

• For instance, in May 2024, QIAGEN, announced the launch of its QIAseq Multimodal DNA/RNA Library Kit, which was a consumable. The new kit enabled seamless preparation of DNA and RNA libraries for next-generation sequencing (NGS), such as whole genome sequencing (WGS) and whole transcriptome sequencing (WTS), and downstream target enrichment based on hybrid-capture from a single sample.

Workflow

On the foundation of workflow, the market is divided into nucleic acid extraction, library preparation and amplification, target enrichment, and library quantification/QC. The library preparation and amplification segment of the workflow holds the highest position in the market as it is an essential step in almost every NGS application, encompassing DNA fragmentation, adapter ligation, and amplification, making it a crucial component of the overall workflow.

• For instance, in March 2024, seqWell has launched the ExpressPlex HT Library Preparation Kit, the first commercially available next-generation sequencing (NGS) library preparation kit. This kit contains all the required reagents and indices for multiplexing up to 6,144 samples in a 384-well format. The 90-minute workflow in a single mastermix reaction streamlines NGS library preparation, enabling lower cost, high throughput sequencing of synthetic constructs used in synthetic biology.

Method

Contingent on method, the market is sub-segmented into microfluidic and automated. The automated NGS sample preparation segment rules over the market dynamics as it has the ability to handle high sample volumes with greater consistency, reduces the manual intervention, and increases the throughput in clinical and research settings, thereby, making it more suitable for large-scale projects.

• For instance, in June 2022, PerkinElmer, Inc., a global leader committed to innovating for a healthier world, announced the launch of the research use only (RUO) BioQule NGS System, which was, an automated benchtop solution for next-generation sequencing (NGS) library preparation of up to eight samples. By incorporating automated thermocycling, integrated quality control through optical quantification and robust liquid handling technology into a single device, the BioQule NGS System enabled researchers to produce high quality NGS libraries that yielded reliable, reproducible results in a variety of applications requiring genomic analysis.

Application

The market is catalogued into oncology, clinical investigation, reproductive health, HLA typing/immune system monitoring, metagenomics, epidemiology and drug development, agrigenomics and forensics, and consumer genomics, on the groundwork of application. The oncology segment is attributed to lead the market as the cases of cancer are rising day by day and there is also a significant focus on personalized medicine in cancer treatment, where NGS plays a crucial role in identifying the genetic mutations to guide targeted therapies.

• For instance, in August 2024, Beckman Coulter Life Sciences, a global leader in laboratory automation and innovation, in partnership with Illumina, a leader in DNA sequencing and array-based technologies, offered a promising new approach to oncology research delivering faster results with fewer touchpoints. The Illumina TruSight Oncology 500 DNA/RNA assay on the Biomek NGeniuS System from Beckman Coulter Life Sciences provided an innovative automated solution enabling comprehensive genomic profiling of tumor samples. The Biomek NGeniuS System was a purpose-built liquid handler for low-medium throughput next generation sequencing (NGS) library preparation.

End-User

According to end user, the market is branched into academic research, clinical research, hospitals and clinics, pharmaceutical and biotechnology entities, and others. The academic research segment governs over the NGS sample preparation market as NGS is used widely in research projects, including disease diagnostics and oncology precision medicine at universities and research centers.

Region

Geographically, the market is classified into North America, Europe, Asia Pacific, Latin America, and the Middle East & Africa.

North America: This region has biggest NGS sample preparation market share. This is due to the region’s advanced healthcare infrastructure, investments in research and development, adoption of NGS technologies in clinical diagnostics, and the growing awareness among the population regarding genetic testing and personalized medicine.

• For instance, in November 2023, New England Biolabs (NEB) has introduced the NEBNext UltraExpress DNA and RNA Library Prep Kits for next-generation sequencing (NGS) on the Illumina platform. These kits offer a faster, easier workflow for generating high-quality, high-yield libraries, allowing RNA library prep in a single day and DNA library prep in under two hours. They are suitable for a wide range of inputs and utilize a single protocol, making them automation-friendly and minimizing consumable waste.

Europe: This region has the fastest growing market over the forecast period. This is due to the region’s strong focus on research and development in genomics, high adoption of NGS technology in clinical diagnostics, a robust healthcare infrastructure, and a growing demand for personalized medicine across the region.

• For instance, in April 2024, NewBiologix SA, a technology innovation company focused on addressing gene therapy manufacturing gaps, announced the launch of its next-generation sequencing (NGS) and optical mapping platform, which was a novel suite of technologies combined to offer comprehensive genomic analysis services to the biopharmaceutical industry. This platform integrated the latest advancements in sequencing and genomic mapping technologies.

NGS Sample Preparation Market Report Scope:

Attribute	Details
Market Size 2025	USD 5.2 Billion
Projected Market Size 2035	USD 19.5 Billion
CAGR Growth Rate	15.6% (2025-2035)
Base year for estimation	2024
Forecast period	2025 – 2035
Market representation	Revenue in USD Billion & CAGR from 2025 to 2035
Regional scope	North America - U.S. and Canada Europe – Germany, U.K., France, Russia, Italy, Spain, Netherlands, and Rest of Europe Asia Pacific – China, India, Japan, Australia, Indonesia Malaysia, South Korea, and Rest of Asia Pacific Latin America - Brazil, Mexico, Argentina, and Rest of Latin America Middle East & Africa – GCC, Israel, South Africa, and Rest of the Middle East & Africa
Report coverage	Revenue forecast, company share, competitive landscape, growth factors, and trends

Segmentation:

By Offering:

• Instruments
  • Automated Liquid Handling Workstations
  • Thermocyclers
  • Others
• Consumables
  • Kits
  • Reagents
  • Media
• Services
  • Library Preparation
  • Sample Extraction
  • Quality Control

By Workflow:

• Nucleic Acid Extraction
• Library Preparation and Amplification
• Target Enrichment
• Library Quantification/QC

By Method:

• Microfluidic
• Automated

By Application:

• Oncology
• Clinical Investigation
• Reproductive Health
• HLA Typing/Immune System Monitoring
• Metagenomics, Epidemiology and Drug Development
• Agrigenomics and Forensics
• Consumer Genomics

By End-User:

• Academic Research
• Clinical Research
• Hospitals and Clinics
• Pharmaceutical and Biotechnology Entities
• Others

By Region:

• North America
  • U.S.
  • Canada
• Europe
  • Germany
  • U.K.
  • France
  • Russia
  • Italy
  • Spain
  • Netherlands
  • Rest of Europe
• Asia Pacific
  • China
  • India
  • Japan
  • Australia
  • Indonesia
  • Malaysia
  • South Korea
  • Rest of Asia Pacific
• Latin America
  • Brazil
  • Mexico
  • Argentina
  • Rest of Latin America
• Middle East & Africa
  • GCC
  • Israel
  • South Africa
  • Rest of the Middle East & Africa

The key players operation in this sector adopt strategies such as, focusing on high-throughput, cost-effectiveness, targeted library preparation, and robust quality control, for fueling the market growth. The companies operating in the market are Illumina, Inc., Thermo Fisher Scientific Inc., QIAGEN, F. Hoffmann-La Roche Ltd, Agilent, and others.

NGS Sample Preparation Market Companies:

• Illumina, Inc.
• Thermo Fisher Scientific Inc.
• QIAGEN
• F. Hoffmann-La Roche Ltd
• Agilent
• PerkinElmer
• Bio-Rad Laboratories, Inc.
• BGI Genomics
• LGC Biosearch Technologies
• Front Line Genomics
• BioChain Institute Inc.
• Beckman Coulter, Inc.
• Merck KGaA
• Promega Corporation

View a Additional List of Companies in NGS Sample Preparation Market

• In September 2023, INTEGRA Biosciences had launched MIRO CANVAS, which was a compact digital microfluidics platform for fully automated NGS sample preparation, to provide a solution that would help accelerate genomics discoveries. MIRO CANVAS had been developed to help solve the common issues faced by researchers when preparing samples for NGS. This revolutionary device performed fully automated library preparation and target enrichment hybridization, drastically simplifying NGS workflows for more walk-away time. These preinstalled workflows ensure accuracy, precision and reliability for high quality end results.
• In June 2024, Beckman Coulter Life Sciences, accelerated high-throughput genomic sample preparation workflows to deliver results faster with the launch of its Biomek Echo One System. The new solution provided versatile integrated acoustic liquid handling options for applications in genomic workflows, including high throughput isothermal DNA assemblies and NGS library sample preparations.

Analyst View:

The NGS sample preparation market is growing due in part to its ability to swiftly and economically sequence large amounts of genetic material, which leads to high throughput sequencing, the detection of uncommon variants, and the flexibility to target particular regions of interest through targeted sequencing. Further driving market expansion are technical developments in NGS platforms, the rising need for NGS in healthcare, its application in genetic testing, and its usage in customized medicine.

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Company Name	Illumina, Inc.
Headquarter	California, U.S.
CEO	Mr. Jacob Thaysen
Employee Count (2024)	10,590 Employees