Competitive Analysis of Top 10 Companies in the Next-Generation Sequencing (NGS) Market 2025
What is Next-Generation Sequencing (NGS)?
Next-generation sequencing (NGS) or massively parallel sequencing is one of the most rapid, high-capacity, and scalable sequencing methods across the globe. This sequencing method is majorly used to ascertain the nucleotide order of specific RNA or DNA portions or the whole genomes. It is a technology that allows researchers for rapid and high-throughput DNA or RNA sequencing, further enabling researchers to significantly study large amounts of genetic material. However, the next-generation sequencing (NGS) technology is not yet being used in routine clinical practice. NGS offers many advantages, such as the ability to study large data sets, cost-effectiveness, and high throughput. This method is highly utilized for forensic science, precision medicine, cancer research, microbial ecology, animal and plant genomics, and epigenetics.
According to Prophecy Market Insights’, Next-Generation Sequencing (NGS) Market report, the market was valued at USD 11.8 Billion in 2024 and is projected to reach USD 37.8 Billion growing at a CAGR of 13.6% by 2034.
The increased genome mapping programs for assigning particular genes to an area of chromosome and also to determine its location and distance between the chromosomes and genes is expected to bolster the market expansion globally. The growth is also observed because of the rising number of NGS applications in various healthcare practice places worldwide.
The wide use of several advanced technologies to collect high-throughput data, further saving the processing time for large amounts of DNA or RNA strands, is also augmenting the market expansion.
Type of Next-Generation Sequencing (NGS):
The types of NGS technology include:
- Whole Genome Sequencing (WGS):
The whole genome sequencing is a process in which the entire genome of an organism is sequenced, further offering a comprehensive view of the organism’s genetic makeover. In this type, the NGS technology platforms has the ability to sequence millions of DNA fragments parallel, enabling cost-effective and rapid whole genome analysis. The WGS is mainly used to identify genetic variations, diagnose genetic diseases, and observe evolutionary relationships.
- RNA Sequencing (RNA-Seq):
RNA sequencing or RNA-Seq refers to a process in which all RNA molecules (transcriptome) are sequenced to examine gene expression and identify various genes. In this process, the RNA is first converted into cDNA, then fragmented, and lastly sequenced using the various next-generation sequencing (NGS) technologies. With is method, researchers can analyze gene expression patterns, understand disease mechanisms, and identify novel RNA molecules.
- Targeted Sequencing:
Targeted sequencing is process in which researchers focus on particular areas of the genome like exons or genes, instead of the entire genome. The targeted sequencing techniques, such as capture or amplification, can enrich for particular areas of interest. It is used to identify mutations in particular genes, study cancer, and also diagnose genetic disorders.
- Single-Molecule Sequencing:
In single-molecule sequencing, the DNA molecules are sequenced one at a time, enabling high accuracy and longer read lengths. These techniques, such as ligation sequencing or nanopore sequencing, are major used to sequence DNA molecules individually. These are helpful in analyzing large genomic regions, detect rare variants, and also identify structural variations.
- Amplicon Sequencing:
Amplicon sequencing is used to sequence particular DNA areas (also known as amplicons) by using PCR. In this technique, PCR amplify the specific DNA regions and then they are sequenced using NGS technology. The technique help researchers identify mutations in specific genes, analyze microbial communities, and also detect pathogens.
Some of the Leading Next-Generation Sequencing (NGS) Offering Companies are:
Hoffmann-La Roche AG (Roche), is a multinational healthcare companies based in Switzerland operating under two divisions, including Diagnostics and Pharmaceuticals, globally. Roche is highly focused on discovering and developing new medicines, along with diagnostics, and digital health solutions, such as NGS, which support in improving healthcare results. Recently, Roche has launched NGS AVENIO Tumor Tissue Analysis Kits to determine solid tumors’ genetic characteristics and oncology research.
Novartis International AG
Based in Basel, Switzerland, Novartis International AG or Novartis is a multinational pharmaceutical company situated in Basel, Switzerland. The innovative medicines of Novartis address the large disease burdens of the society and restore possibility for patients. The next-generation sequencing-based test by Genoptix, a California-based Novartis molecular diagnostics firm is helping researchers to diagnose non-small cell lung cancer.
Johnson & Johnson
Founded in January 1886, Johnson & Johnson is a New Jersey, U.S.-based multinational, medical technologies, pharmaceutical, and biotechnology company. The company is leveraging the NGS technology, specifically via its Janssen Diagnostics division for improving its research and diagnostics, such as cancer and many other rare diseases for developing ways for precision medicine.
Established in 1849, Pfizer Inc. is a U.S.-based multinational pharmaceutical and biotechnology company, which is headquartered in Manhattan, New York City. The company develops and manufacture vaccines and medicines to treat diseases related to oncology, cardiology, immunology, neurology, and endocrinology. On May 9, 2023, Pfizer signed an agreement to increase local access to NGS-based testing for patients suffering from lung and breast cancers in over 30 countries worldwide.
Merck & Co., Inc. is a U.S.-based multinational pharmaceutical company built in 1891, which is a trade name for Merck Group, established in Germany in 1668. Merck develop and produce vaccines, medicines, animal health products, and biologic therapies. The company is largely supporting different NGS applications like deciphering genetic biomarkers, single-cell sequencing, gene target resequencing, and many others.
Browse through the Additional List of Companies in the Next-Generation Sequencing (NGS) Market
How Government-Aided Genome Projects are Boosting the Implementation of Next-Generation Sequencing (NGS) Technology?
Next-generation sequencing is playing an important roles in the process of disease diagnosis. The disease diagnosis and management’s landscape has changed significantly in the past decade because of the governments across the globe have begun investing a substantial amount in genomic projects. These genomic projects helps in raising funds, developing infrastructure, standardization, reduction in cost, building comprehensive genomic databases, increasing healthcare, and biomedical research. These government-supported projects are also beneficial for worldwide collaborations and data sharing, workforce development, training, support public health initiatives and agriculture and environmental science, and also encourage investments in the NGS industry.